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Another object of this invention is to provide an affinity carrier which exhibits a high pressure-resistant strength when filled in a column and can treat treatment liquors under pressure at a high flowing velocity through the column, and has a high treating ability.
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Still another feature of the affinity carrier is that it has extremely low nonspecific absorption of components other than that which is to be separated. Hitachi Content Platform. Preferably, the fine particles of ion-exchanged cellulose of this invention further have the following properties. The reaction mixture was transferred to a glass filter, washed with 30 ml of 0. If required, to remove the remaining alkali, the particles are then washed with an acid such as dilute hydrochloric acid, water or methanol.
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Step A of preparing an alkaline aqueous solution of cellulose xanthate and the first water-soluble polymeric compound other than cellulose xanthate is carried out in the same way as the first method of production described above.
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|The cellulose xanthate was dissolved in an aqueous solution of sodium hydroxide, and g of flakes of polyethylene glycol molecular weight 20, were added and dissolved to prepare an alkaline aqueous polymeric solution of cellulose xanthate and polyethylene glycol.
The cellulose particles were activated at a pH of 11 with cyanogen bromide in an amount corresponding to three equivalents per equivalent of the carbohydrate unit structure, and reacted with 0.
The infrared absorption spectrum of the tablets was measured by the transmission method. By the second method, the alkaline aqueous solution of polymers is mixed with the water-soluble high-molecular-weight polyethylene glycol or its derivative in step B. The affinity carrier of claim 1 in which the total volume of pores having a pore diameter in the range of 0.
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The properties of the resulting ion-exchanged cellulose particles are shown in Table 8, Run No. Removal of the polymeric compounds in the fine coagulated viscose particles is carried out with 0. The wet pressure resistances and the amounts of BSA introduced of these particles are shown in Table Hitachi Content Intelligence.
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Accordingly, another characteristic is that the affinity carrier of the invention can be used at a high flow rate as required. The affinity separating technique utilizing specific affinitive powder between biological substances has widely been used for the purpose of specifically adsorbing trace substances produced in a biological system and separate and purify them, or removing a specific component from a plasma preparation.
This led to the determination that when the above ion-exchanged cellulose particles are filled in a column, and ion-exchange chromatography is carried out, the cellulose particles have excellent flowing liquid property and pressure resistance. Porous ion-exchanged fine cellulose particles, method for production thereof, and affinity carrier.
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at various magnifica- tions at (A) ×, (B) × and (C) ×. (c) batch and a slight decrease in weight was observed.
According to the first method, an alkaline aqueous solution of cellulose xanthate and a first water-soluble polymeric compound other than cellulose xanthate is prepared in step A, and in step B, a dispersion of fine particles of the alkaline aqueous solution of polymers is prepared. Advanced Metadata Features and Tools Implement your big data strategy by tagging data with custom metadata to manage and search trillions of file objects.
Critical drying method To determine accurately the porous property of cellulose particles swollen with water, the structure of cellulose was fixed by the critical drying method while retaining the swollen state. Cellulose xanthate obtained in the same way as in Example 1 was dissolved in an aqueous solution of sodium hydroxide, and then polyethylene glycol molecular weight 4, was added in an amount of g, g and 1, g, respectively to give porous fine cellulose particles.
The amount of the remaining BSA in the solution was measured to determine the amount of bound BSA per unit amount of dry particles.
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|Passivated porous polymer supports and methods for the preparation and use of same.
Visit Us. Calculation of the ion-exchange capacity About 1 g of the dried cellulose particles was precisely weighed. Accordingly, another characteristic is that the affinity carrier of the invention can be used at a high flow rate as required.
This exclusion limit molecular weight is determined by using polyethylene glycol as a high-molecular-weight substance. In the treatment 3the regenerated fine cellulose particles are crosslinked in a liquid medium containing an alkali hydroxide.